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Comparison of NIRS and other methods to determine anaerobic threshold
Šimon, Jan ; Slabý, Kryštof (advisor) ; Dostal, Jiří (referee)
The aim of this study was to compare NIRS (Near-infrared spectroscopy) and respiratory parameters measurement in determination of anaerobic treshold. Additionally changes in muscle oxygenation (SmO2) were described during incremental bicycle excercise. Fifteen subjects, 9 men and 6 women took part in this study. To verify the reliability of the measurement, the experiment was performed in the form of a test/retest with a few day's delay. The value of SmO2 was measured in m. gastrocnemius lateralis and m. vastus lateralis. In addition, respiratory parameters and pulse rate were recorded during the examination. The data obtained show that the breakpoint in the SmO2 dynamics determined by the NIRS method means that the excercise intensity above the anaerobic threshold is reached, because the breakpoint is located in the area of the Respiratory Compensation Point. In the SmO2 parameter, it is also possible to monitor the equilibrium state during the rest phase. The SmO2 parameter after the ramp log terminates exceeds the value in the rest phase, and returns to the quiescent value usually within 2 minutes. The test/re-test method has verified the reliability of the measurement.
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Analysis of urine after dynamic load with mean attention on tubular markers
Hrstková, Vladěna ; Procházka, Michal (advisor) ; Bitnar, Petr (referee)
The main focus of our research was to compare changes in ion excretion in urine, osmolality, indicators of exercise-induced proteinuria and tubular markers before and after dynamic exercise on a bicycle ergometer. The test sample consisted of 7 healthy, physically active women aged 27.4 years ( σ = 3.82 ). For dynamic exercise we uniformly set parameters 65 % VO2max and duration of load in 50 minutes. We compared the laboratory results of urine samples taken just before the exercise with urinary samples that we have collected immediately after exercise. The results of our measurements showed a significant changes in exercise-induced urinary sodium ions, creatinine and phosphorus. Statistically insignificant changes excretion was found between markers: chlorine, urine, osmolality, and N-acetyl-beta- d-glucosaminidase. These markers, however, show a trend the significance. Statistically insignificant changes after exercise have shown excretion of urinary markers: potassium, gelatinase-associated lipocalin, neutrophil microalbuminuria and alpha-1-microglobulin. Tubular markers of kidney damage after exercise is not at the reference values, that would indicate the tubular kidney damage.
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